Journal: Cell reports

Article Title: T cell-specific P2RX7 favors lung parenchymal CD4 + T cell accumulation in response to severe lung infections

doi: 10.1016/j.celrep.2023.113448

Figure Lengend Snippet: CD4-KO ( Cd4 −/− ) mice were infected with ~100 CFU of Mbv MP287. Non-infected and infected CD4-KO mice were used as controls. At day 5 p.i., mice received splenic CD4 + T cells (1 × 10 6 ) from WT and P2RX7-KO ( P2rx7 −/− ) mice. (A) Experimental protocol ( BioRender.com ). (B) Average body weights (percentages related to day 0). (C) Lung weights/mice. (D) Lung CFU numbers. (E) Macroscopic images of right upper lung lobes. Scale bar, 1 cm. (F) Representative lung sections stained with H&E. Scale bars, 500 μm. (G) Left: flow-cytometry plots of CD44 and CD45i.v. on CD4 + T cells. Right: average frequencies of CD45i.v. + and CD45i.v. − cells in CD44 + CD4 + T cells. (H) Average numbers of CD44 + CD4 + T cells in the medLNs. (I) Representative confocal images of CD4, IFN-γ, and S100A9 expression in infected lung tissues. Scale bars, 50 μm. Data from three independent experiments, n = 3–5/experimental group per experiment. Data shown as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA (Tukey post tests) (C, D, and G) or unpaired t tests (B and H).

Article Snippet: Subsequently, the sections were blocked with 2% BSA in PBS for 30 min, and then incubated overnight at 4°C with primary anti-S100A9 antibody (Bio-Techne).

Techniques: Infection, Staining, Flow Cytometry, Expressing

Journal: Cell reports

Article Title: T cell-specific P2RX7 favors lung parenchymal CD4 + T cell accumulation in response to severe lung infections

doi: 10.1016/j.celrep.2023.113448

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Subsequently, the sections were blocked with 2% BSA in PBS for 30 min, and then incubated overnight at 4°C with primary anti-S100A9 antibody (Bio-Techne).

Techniques: Virus, Recombinant, Saline, Cell Isolation, Isolation, Software

Journal: Microorganisms

Article Title: Draft Genome of Nocardia canadensis sp. nov. Isolated from Petroleum-Hydrocarbon-Contaminated Soil

doi: 10.3390/microorganisms11122972

Figure Lengend Snippet: Map of the scaffolded contigs of the Nocardia strain WB46. From outer to inner ring: the individual contigs (blue arrows), scale, coding sequences (green) on the forward strand and reverse strand, pseudogenes (purple) on the forward strand and reverse strand, RNA genes on the forward strand and reverse strand (tRNAs green, rRNAs red, other RNAs blue), G + C content (black), and CG-skew (orange).

Article Snippet: The Nocardia sp. strain WB46 was also uploaded to the Type (Strain) Genome Server (TYGS) ( https://tygs.dsmz.de , accessed on 11 December 2023) for a whole-genome-based taxonomic analysis.

Techniques:

Journal: Microorganisms

Article Title: Draft Genome of Nocardia canadensis sp. nov. Isolated from Petroleum-Hydrocarbon-Contaminated Soil

doi: 10.3390/microorganisms11122972

Figure Lengend Snippet: Phylogenetic analysis of Nocardia sp. strain WB46 with other species in the Nocardia genus, using complete 16S rRNA gene sequences (1358 bp), was conducted using Bayesian phylogenetic analysis. The GTR + I + G (with four distinct gamma categories) phylogenetic model showed the lowest BIC value. The tree was rooted using Rhodococcus equi as an outgroup (colored orange), following the previous publication of Nocardia phylogeny . The numbers at branches correspond to Bayesian posterior probabilities. The branches of a clade ( N. asteroides ), which are suggested to share the most direct common ancestor with Nocardia sp. WB46 (colored red) with 1.0/1.0 posterior probability, are colored red.

Article Snippet: The Nocardia sp. strain WB46 was also uploaded to the Type (Strain) Genome Server (TYGS) ( https://tygs.dsmz.de , accessed on 11 December 2023) for a whole-genome-based taxonomic analysis.

Techniques:

Journal: Microorganisms

Article Title: Draft Genome of Nocardia canadensis sp. nov. Isolated from Petroleum-Hydrocarbon-Contaminated Soil

doi: 10.3390/microorganisms11122972

Figure Lengend Snippet: Region of 16S rDNA sequence divergence in multiple sequence alignment of Nocardia sp. strain WB46 and Nocardia asteroides isolates was observed. Among the full-length 16S rDNA sequence, 6 nucleotide sequences differed in Nocardia sp. strain WB46 and the other Nocardia asteroides isolates with no sequence divergence. The diverged sequences are shown without a background color.

Article Snippet: The Nocardia sp. strain WB46 was also uploaded to the Type (Strain) Genome Server (TYGS) ( https://tygs.dsmz.de , accessed on 11 December 2023) for a whole-genome-based taxonomic analysis.

Techniques: Sequencing